Induction of animal model of Graves disease in BALB/c mice

Objective To construct an animal model of Graves' disease (GD) by immunizing BALB/c mice with hM12 cells co-expressing major histocompatibility complex (MHC) class Ⅱ molecules and human thyrotropin receptor (TSHR) molecules. Methods BALB/c mice in experimental group (H-2d) were immunized with hM12 cells Intraper-itoncally every 2 weeks for six times, while mice in control group were immunized with M12 cells. Five weeks later, the thyroids were histologically examined, and serum samples were tested for thyroid-stimulating antibodies (TSAb) and thyroid hormone levels. Results One BALB/c mouse in experimental group developed Graves'-like disease. Total T4 and T3 levels in this mouse were above the upper limit of normal, TSAb activity was displayed in its serum. The thyroid histologically showed the features of thyroid hyperactivity including thyrocyte hypercellularity and colloid absorption.None of control mice developed Graves'-like disease. Conclusion An animal model with some characteristics of human Graves' disease was successfully induced and the model will facilitate studies aimed directly at understanding the patho-genesis of autoimmunity in Graves' disease.

作 者： Zhu-fang Tian Bing-yin Shi Xiao-yan Wu Li Xu   作者單位： Zhu-fang Tian(Department of Endocrinology, the First Affiliated Hospital, Medical School of Xi'an Jiaotong Uni-versity, Xi'an 710061;Department of Endocrinology, Xi'an Central Hospital, Xi'an 710003, China)

Bing-yin Shi,Xiao-yan Wu,Li Xu(Department of Endocrinology, the First Affiliated Hospital, Medical School of Xi'an Jiaotong Uni-versity, Xi'an 710061) 

刊 名： 西安交通大學(xué)學(xué)報(bào)（英文版）  英文刊名： ACADEMIC JOURNAL OF XI'AN JIAOTONG UNIVERSITY(ENGLISH EDITION)  年，卷(期)： 2009 21(4)  分類號(hào)： Q786 R347.514  關(guān)鍵詞： major histocompatibility complex Ⅱ   thyrotropin receptor   M12 cell   Graves' disease  

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