Electrochemical Study on the Interaction Betwwen Neutral Red and DNA

A voltammetric study of the interaction of neutral Red(NR) with DNA at a gold electrode in a phosphate buffer solution is described. After adding DNA in an NR solution, the reduction peak current of NR decreases. The binding mechahisms of NR to DNA in different pH ranges are different. The reduction peak potential of NR in a pH 7.0 phosphate buffer solution in the presence of DNA shifts positively, indicating that the binding of NR to DNA is intercalation action, but at pH=6.0 the reduction peak potential of NR shifts negatively, indicating that the binding of NR to DNA is electrostatic action. The formed complexes are DNA-NR when [NR]/[DNA]<0.18 and DNA-3NR when [NR]/[DNA]>0.35, respectively.

作 者： NIU Shu-yan ZHANG Shu-sheng SHI Xin JIAO Kui   作者單位： NIU Shu-yan(College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, P. R. China;College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China)

ZHANG Shu-sheng,SHI Xin,JIAO Kui(College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China) 

刊 名： 高等學(xué)�；瘜W(xué)研究（英文版）  ISTIC SCI 英文刊名： CHEMICAL RESEARCH IN CHINESE UNIVERSITIES  年，卷(期)： 2005 21(1)  分類(lèi)號(hào)： O6  關(guān)鍵詞： Neutral Red   DNA   Cyclic voltammetry   Intercalation  

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